Pathway-based comparative proteomics of human somatic cells, induced pluripotent stem cells, and embryonic stem cells

• Main Authors: LUI SHOOK MUN WINIE, 呂淑雯
• Other Authors: Kun-Pin Wu
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/49546616452385164022

碩士 === 國立陽明大學 === 生物醫學資訊研究所 === 101 === Owing to the clinical potential of hiPSCs (human induced pluripotent stem cells) in regenerative medicine, a thorough examination on the similarities and differences between hiPSCs and hESCs (human embryonic stem cells) has become indispensable. In this study, a comparative pathway analysis by using the bioinformatics tool GSEA was performed on the dataset of membrane proteomics analysis of hiPSCs, hESCs, and HFFs (precursor human foreskin fibroblast) cells. Based on the relative expression profiles in the three pairwise comparisons (hiPSCs vs hESCs; hESCs vs HFF; hiPSCs vs HFF), we aimed at elucidating the reprogramming degree of hiPSCs in-between HFF and hESCs. Our results revealed that pathways related to electron transport and oxidative phosphorylation and related to focal adhesion were significant enriched and might be contribute to incomplete reprogramming within hiPSCs. The identified pathways and the core enriched proteins contribute to the pathways may serve as candidates for exploring the molecular mechanism that can improve the process of hiPSCs reprogramming.