Raman spectroscopy for urine crystals detection with magnetic nanobeads separation technique

• Main Authors: Po-An Chen, 陳柏安
• Other Authors: Huihua Kenny Chiang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/53255309177049849817

碩士 === 國立陽明大學 === 生醫光電研究所 === 101 === Urolithiasis is a common disease with high recurrence rate. The prevalence rate of Urolithiasis is about 4-15% in Asia, Europe and America. The recurrence rate of Urolithiasis is up to 50% after the treatment. It is important to analyze the compound of urinary stones accurately for prevenion of new stone formation. Identification of urinary stone composition is important for metabolic evaluation, decision-making and diet control. Stones are composed of crystals in the urinary system, previous study discover crystals will appear in the urine of patients. In this study, we develop the magnetic nanomaterial that target to crystals chemical bond by hydrothermal method, and with phosphorylate-terminated surface by (EDC)1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride / (NHS) N-Hydroxysuccinimide technique. By using an external magnetic field to separate Fe3O4-Crystals from urine. The sample substrate is placed into the detection system analysis. We can quickly analysis the crystal species by Raman. Then, we can predict the stone composition. Raman scattering is a typical molecular scattering phenomenon, representing the vibrational and rotational energy distribution of molecules; it has a narrow emission band, and has advantages in being a molecular specific spectrum, direct measurement, qualitative and quantitative measurement characteristics. Last, we can predict the stone composition, improve the detection efficiency, and reduce error judgment with microscopy. The technology is convenient, rapid, and accurate. This qualitative method can replace the traditional approach.