Using light sheet microscopy to image 3D scaffold

• Main Authors: Chi-An Huang, 黃麒安
• Other Authors: Chau-Hwang Lee
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/33657553090761617355

碩士 === 國立陽明大學 === 生醫光電研究所 === 101 === Fluorescence microscopy has provided valuable information in biology research. One of the most powerful fluorescence microscopes to date is the confocal microscope. However, the axial resolution is reduces with depth increases due to spherical aberration which limits the size of object that can be studied. In this study, we constructed a light sheet microscope with 1.3 m lateral resolution and 10.7 m axial resolution which is able to image sample in the depth of 700m and we also measure the point spread function with the changes of depth.