| Summary: | 博士 === 國立陽明大學 === 生化暨分子生物研究所 === 101 === Lung stem/progenitor cells (LSCs) play protective roles in epithelial repair and tissue homeostasis. However, isolation of cells is challenging, and little is known about their homing capacity in vivo. Using a new glycoproteomic technique, we discovered a specific glycoprotein marker, CD157, which together with CD45 and CD54 enables the isolation of murine and human LSCs by fluorescence-activated cell sorting (FACS). The isolated cells are able to undergo self-renewal, expansion, and sequential differentiation into type II and then type I pneumocytes. Here we show that fluorescent nanodiamonds (FND), in combination with FACS, fluorescence lifetime imaging microscopy and immunostaining, can identify transplanted CD45–CD54+CD157+ LSCs in vivo, and track their engraftment and regenerative capabilities with single-cell resolution. FND labeling did not eliminate the cells properties of self-renewal and differentiation. Time-gated fluorescence imaging of tissue sections of naphthalene-injured mice indicates that the FND-labeled LSCs preferentially reside at terminal bronchioles after intravenous transplantation. In addition, these cells are susceptible to both influenza virus and SARS-CoV infection, they may serve as a novel cell-based system for viral infection, pathogenesis, drug screening and further studies of regenerative medicine and stem cell therapy.
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