The function of progesterone receptor in zebrafish embryo dorsal-ventral patterning

• Main Authors: Tsung-Yen Huang, 黃從晏
• Other Authors: Bon-Chu Chung
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/13194436195769793730

碩士 === 國立陽明大學 === 生命科學系暨基因體科學研究所 === 101 === Steroid hormones regulate a variety of physiological responses and embryo development. 3beta-hydroxysteroid dehydrogenase (Hsd3b) is a crucial enzyme that catalyzed the synthesis of progesterone. Depletion of hsd3b2 causes zebrafish embryo dorsalization and decreased bmp4 expression. I tested the hypothesis that whether Hsd3b2 regulates bmp4 expression through progesterone receptor (Pgr) in zebrafish. Using a morpholino to block pgr expression in zebrafish embryo, I found dorsalization morphology in embryos. Both the bmp4 expression level and expression domain were reduced in the pgr morphants, whereas no obvious abnormality had been discovered in bmp2b and bmp7 expression in the pgr morphants during late blastula to early gastrula stage. The amount of phosphorylated-Smads was also decreased in the pgr morphants after late blastula stage. These results suggested that progesterone receptor may involve in the dorsoventral patterning of zebrafish embryo through Bmp4 signaling pathway.