Injectable in Situ Forming pH-response Hydrogel as Delivery Vehicle for Cartilage Tissue Engineering

• Main Authors: Hsing-Yi Yu, 余星億
• Other Authors: Hsi-Chin Wu
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/52535086530033622095

碩士 === 大同大學 === 材料工程學系(所) === 101 === Injectable hydrogels do not require a surgical procedure for implantation, and various therapeutic drugs can be incorporated through simple mixing, due the sol - gel state conversion characteristics. Preparation of pH-sensitive hygrogel in this study, the use of chitosan modified, prepared as a N,O carboxymethyl chitosan（NOCC）and hyaluronic acid （HA） derivative aldehyded hyaluronic acid （AHA）, via schiff base reaction. FTIR analysis, observation chitosan successfully modified N,O carboxymethyl chitosan（NOCC）. TNBSA analysis, determination of the degree of the oxide hyaluronic acid and modified degree of N,O carboxymethyl chitosan（NOCC）. Rheological Behavior analysis, rate hydrogel gelling point and intensity of hydrogel. Situ forming hydrogel NOCC / AHA from the form of a liquid into a gel state about within 25 seconds -11 minutes, with NOCC concentration increases gelling time shorter, due NOCC concentration improve hove more amine group （NH2） with AHA have aldehyde group （CHO） crosslink. SEM analysis, observed NOCC concentration increases have smaller pores. Drug release study, pH 4.5 lower drug release capability with pH 7.4 higher drug release capability, but NOCC concentration improve have a lower drug release capability, due NOCC concentration have impact the pores of the hydrogel. In brief, an injectable NOCC/AHA pH-sensitive hydrogel system was successfully developed for in situ local controlled release as novel drug carrier for biomedical applications.