| Summary: | 碩士 === 國立臺北科技大學 === 生化與生醫工程研究所 === 101 === The lack of ideal biomarkers for Cholangiocarcinoma (CCA) diagnosis, prognosis, and therapy, therefore result in CCA is a lethal disease, with the 5-year survival rates being less than 5%. To reveal pathogenesis of CCA may help with clinical considerations in diagnosis and treatment. Herein, thioacetamide (TAA)-induced Male Sprague-Dawley (SD) rats CCA model were used in current study. We aim to study the cytogenetic features of CCA tumor development by using rats CCA model. We established CGCCA cell line and observed ring and giant rod mark chromosomes；And we observed genes involced in biological function and associtated with CCA by gene expression micfroarray；We used serial different weeks rat CCA for array comparative genomic hybridization to identify copy number variations and candidate genes, +2q45, +Xq22, -12p12 have been identified for the tumor early stage, where involve the gene TNNI3K. In addition, 16 genes and 3 loci were associated with rapid tumor progression; Chemokine signaling pathway、ERBB signaling pathway、JAK-STAT signaling pathway、MAPK signaling pathway、VEGF signaling pathway were highly suggest correlated to late stage of CCA. It might uncover the mysterious about CCA pathogenesis, and provide the potential mechanism to the clinicians for the treatment.
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