The development of antimicrobial mouthwash without alcohol and antibacteria agent addition

• Main Authors: Hsuan-Yu Chen, 陳宣伃
• Other Authors: 方旭偉
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/p4yv39

碩士 === 國立臺北科技大學 === 生化與生醫工程研究所 === 101 === Human can not have regular oral health care, and thus use mouthwash instead. Mouthwash market variety, one of the major antibacterial ingredients for alcohol, fluorine compounds, Chlorhexidine, but these ingredients have prolonged use of secondary damage to human oral health. In order to reduce the harmful microbes and supersedes oral antibacterial ingredients. This study used γ-PGA (γ-Polyglutamic acid) instead of mouthwash ingredients its market to stimulate antibacterial to achieve the effect of oral cleanliness. To show the antibacterial effect, we compared the different brands of commercially antibacterial mouthwash with different concentrations of γ-PGA in 10000ppm have 98% antibacterial effect and antibacterial effect similar to commercial mouthwash.The result confirm add γ-PGA in mouthwash as antibacterial ingredient is effective. By the WST-8 and LDH test result, commercial mouthwash show low cell survival rate. Because of their composition have high levels of alcohol, various essential oils and CHX compound that as the main antibacterial ingredients. We use γ-PGA as the antibacterial ingredient, it’s no harm to human body and no genotoxic. In genotoxic test show mouthwash containing γ-PGA don’t cause DNA damage. Therefor, we develop a antibacterial mouthwash without alcohol and antibacterial agent. It has the potential to replace commercial mouthwash in the future.