Enzymatic production of xylooligosaccharides from napier grass xylan extracted using steam explosion

碩士 === 國立臺北科技大學 === 有機高分子研究所 === 101 === Napiergrass (Pennisetum purpureum) is regarded as a potential crop because its rapid growth rate and high dry matter productivity. In Taiwan, napiergrass juice prevails as a functional juice due to its beneficial health effects. In addition, the residual fibe...

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Bibliographic Details
Main Authors: Teng-Yuan Hsu, 許騰元
Other Authors: 余琬琴
Format: Others
Language:zh-TW
Published: 2013
Online Access:http://ndltd.ncl.edu.tw/handle/ve6cjs
Description
Summary:碩士 === 國立臺北科技大學 === 有機高分子研究所 === 101 === Napiergrass (Pennisetum purpureum) is regarded as a potential crop because its rapid growth rate and high dry matter productivity. In Taiwan, napiergrass juice prevails as a functional juice due to its beneficial health effects. In addition, the residual fiber could be subjected to suitable treatment to increase its economic value, such as production of xylooligosaccharide. In this research, the alkaline steam explosion was used to extract xylan from napiergrass. However, the extracted xylan had a high degree of polymerization. The xylan was degraded via enzymatic hydrolysis to produce xylooligosaccharides. The commercial endo-xylanase was used for decreasing the production of monosaccharide. The effect of pH value and enzyme dosage on xylooligosaccharide production was investigated. Furthermore, the enzymatic hydrolysis result of xylan from alkaline extraction would be investigated. The result showed that if the pH value was lower than 5, the hydrolysis efficiency would be better. When the cost of chemical and enzyme were considered, the ideal hydrolysis conditions were pH 5 with enzyme dose 87.5 U. For comparison of hydrolysis efficiency between alkaline steam explosion and alkaline extraction, the former was preferred due to its higher yield of xylooligosaccharides.