Preparation and characterization of cross-linked gamma-poly glutamate/gelatin hydrogel

• Main Authors: Tsung-Han Lee, 李宗翰
• Other Authors: 鍾仁傑
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/hmh62p

碩士 === 國立臺北科技大學 === 化學工程研究所 === 101 === The healing of wounds on skin is accelerated under a proper humid environment than a dry one. Nevertheless, immoderate free radicals will lead to excessive inflammation reactions and retard the healing processes. The motivation of this study is to prepare a cross-linked composite hydrogel with combining the advantages of gelation gelatin, humidifying γ-poly glutamate (γ-PGA) and antioxidative proanthocyanidins (OPCs). The natural polymer made hydrogel is proposed to be applied as a wound dressing. In this research, the hydrogel wound dressing was prepared by using sodium/calcium γ-PGA and gelatin cross-linked with OPCs. The optimized ratio of γ-PGA salt to gelatin was evaluated through swelling and degradation tests. Furthermore, the hydrogel was cross-linked with OPCs and then tested in vitro and in vivo. Results indicated that the cross-linked hydrogel using OPCs solution had stronger machine structure and improved antioxidant capacity significantly. The results of in vitro tests showed that the cross-linked hydrogel using 10% OPCs solution would be no bio-toxic for L929 cell. The results of in vivo tests showed that the cross-linked hydrogel using 10% OPCs solution would be of great potential to serve as a useful wound healing dressings.