| Summary: | 碩士 === 國立臺北科技大學 === 化學工程研究所 === 101 === UHMWPE wear debris could result in aseptic loosening of components after long-term use of artificial joint replacements. Lubricating fluid plays an important role in the contact mechanism of interfaces. Therefore, in order to extend the life of artificial joints, based on the need to understand the molecular mechanism of lubrication. Variety compositions of joint synovial fluid, among albumin is the most abundant protein.
In this study, investigated the lubrication behavior of thermal human serum albumin (HSA) and hyaluronic acid (HA) interaction by using pin-on-disc friction test and CD Spectroscopy and fluorescence isothiocyanate (FITC) labeled albumin adsorption and rheological properties. Experimental results single molecules system of albumin friction coefficient increased with temperature increased, HA friction coefficient independent temperature. We observed thermal denaturation of albumin adsorption amount, and α-helix structure loss lead to increase friction coefficient. In addition, we further explore HA added to albumin lubrication. Results show,as albumin and hyaluronic acid mixed after thermal treatment reduced friction coefficient. Albumin observed conformational changes influence friction coefficient is not the main reason. The Adsorption amount and viscosity increase lead to reduce friction coefficient.
In the future, we still need study lubrication mechanism between components of synovial fluid and additives. Assist to develop more effective lubricating of biomolecules additives lead to reduce friction and improve lubrication, in order to prolong lifetime of artificial joints.
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