Fabrication of bi-layer collagen cartilage scaffolds by electrospinning and freeze-drying and comparison of the difference between the random and the aligned nano-fibers

• Main Authors: Wen-Chi Tsai, 蔡文啟
• Other Authors: 林忻怡
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/x6fwrw

碩士 === 國立臺北科技大學 === 化學工程研究所 === 101 === 　　Tissue engineering of cartilage is emerging as a technique for the regeneration or repair of damaged cartilage tissue. Tissue engineering has been a feasible way to regenerate cartilage in vitro. The fundamental concept of tissue engineering includes cell isolating, cell culturing in vitro, and implanting in vivo. To obtain nearly native articular cartilage tissue and repair the damaged section are currently the main purposes of cartilage tissue engineering. 　　Articular cartilage tissue is composed of chondrocytes and extracellular matrix, where the chondrocytes only make up about 5% of the total volume of cartilage. Articular cartilage is comprised of four different layers that can be distinguished from one another by the morphology, distribution and density of chondrocytes. The matrix is composed of collagens, especially type II collagen, and proteoglycan. The proliferation and self-repair of articular cartilage are both poor, because chondrocytes can not migrate to the damaged tissue to repair effectively when injured. So, repair the damaged cartilage tissue effectively by the techniques of tissue engineering is the purpose of this study. 　　In this study, we fabricate the porous scaffolds base on collagen for imitating native cartilage tissue by freeze-drying and electrospinning, and cross-link with glutaraldehyde to raise the mechanical properties. And explore the influence of repair of cartilage tissue by physical properties tests and in vitro cell culturing tests. The results indicate that the scaffolds have stable structure and mechanical strength, and in vitro cell culturing tests showed that the scaffolds are able to effectively promote cells attachment and proliferation, and with good biocompatibility.