In-vitro Prevention of Salmonella Lipopolysaccharide-induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains

• Main Authors: Chun-Yan Yeung, 楊俊仁
• Other Authors: Hsuan-Liang Liu
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/82mpad

博士 === 國立臺北科技大學 === 工程科技研究所 === 101 === Lactobacillus shows beneficial anti-inflammatory effects to Salmonella infection. The maintenance of the tight junction (TJ) integrity plays an importance role in avoiding bacterial invasion. Whether Lactobacillus could be used to regulate the TJ protein expression and distribution in inflamed intestinal epithelial cells was determined. Using the transwell co-culture model, Salmonella lipopolysaccharide (LPS) was apically added to polarized Caco-2 cells co-cultured with peripheral blood mononuclear cells in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with various Lactobacillus strains. TJ integrity was determined by measuring trans-epithelial electrical resistance across Caco-2 monolayer. Expression and localization of TJ proteins (zonula occludens (ZO)-1) were determined by western blot and immunofluorescence microscopy. Various strains of Lactobacillus were responsible for the different modulations of cell layer integrity. LPS was specifically able to disrupt epithelial barrier and change the location of ZO-1. Our data demonstrate that Lactobacillus could attenuate the barrier disruption of intestinal epithelial cells caused by Salmonella LPS administration. We showed that Lactobacillus strains are associated with the maintenance of the tight junction integrity and appearance. In this study we provide insight that live probiotics could improve epithelial barrier properties and this may explain the potential mechanism behind to their beneficial effect in-vivo.