Salmonella detection using 16S ribosomal DNA/RNA probe-gold nanoparticles and lateral flow immunoassay

• Main Authors: Cheng-Che Liu, 劉正哲
• Other Authors: Shao-Yi Hou
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/gxy69k

博士 === 國立臺北科技大學 === 工程科技研究所 === 101 === We develop an ultrasensitive, simple, and fast lateral flow immunoassay for Salmonella detection using gold nanoparticles conjugated with a DNA probe, which is complementary to the 16S ribosomal RNA and DNA of Salmonella. The detection limit is 5 fmol for the synthetic single-stranded DNA. For Salmonella cultured samples, the nucleic acids from 107 bacteria were rapidly detected in 30 minutes. After silver enhancement, the detection limit was as low as 104 cells which is lower than 105 bacteria cells, the human infective dose of food-borne Salmonella. Furthermore, the probes used in this study are specific to Salmonella compared to 9 other species of Enterobacteriaceae. This approach would be useful for microbial detection in regards to food safety or clinical diagnosis. It is also suitable for large-scale screening in developing countries because it is sensitive, specific and convenient.