| Summary: | 博士 === 國立臺北科技大學 === 工程科技研究所 === 101 === 1. Chitosan scaffolds effect extracellular matrix expression
Cellular adhesiveness to biomaterial is one of the important properties to the success of tissue engineering. The cell-biomaterial interactions involve close cooperation of adhesion proteins, the plasma membrane, and cytoskeletons in order to form focal adhesions during the process of anchoring. Dynamic development of the plasma membrane in the process reflects the cellular biocompatibility and motility. The process of cell attachment beginning from seeding, contact, attachment, spreading has not been investigated. In this study, we monitored the whole process of cells attaching to the substrate surface by time-lapse confocal microscopy. We observed that the surface configuration of the substratum effects plasma membrane expansion and genomic materials distribution. In contrast to the cells grown on the plate, the cells attached on pillars are with rounded nuclei and with prominent
lamellipodia spreading out. Membrane expansion is involved in dynamic development of the plasma membrane and lamellipodia formation for attachment, migration or proliferation and reflects the cellular physiology status of the cells. This study provides a platform for investigation of cell behavior and dynamic development of subcellular structures regarding cell-biomaterial interactions
2. Cellular behavior and substrate configuration
Chitosan is a choice material for scaffolds in regenerative medicine. One of the applications is to bridge the damaged peripheral nerves. Previous studies showed that combination of chitosan conduits and cultured Schwann cells could increase the opportunity for re-connection of broken nerves. It has also been known that Schwann cells can produce the ECM components which are critical for nerve regeneration. In this study, we used the rat Schwann cells ( RSCs ) grown on porous chitosan scaffolds for quantitative analysis of ECM protein expression. The RSCs grown on chitosan scaffolds secreted higher amount of laminin and collagen 4 than those grown on the plan. The increased laminin and collagen 4 produced by Schwann cells could create a preferable condition for stimulating peripheral nerve regeneration.
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