The Function of MOB2 Protein in Cancer Metastasis

• Main Authors: Ching-te Wu, 吳清德
• Other Authors: Seng-Sheen Fan
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/03748838086069095529

碩士 === 東海大學 === 生命科學系 === 101 === Cell migration is an important biologic function. It participates in several biologic processes, such as embryo development, organization, wound healing, immune reaction and metastasis. Metastasis is the main cause of cancer mortality. Thus, understanding the molecular regulation of cell migration may help to provide a clue to prevent cancer metastasis. It has been known that MOB2 proteins regulate cell polarity and cell morphology in yeasts, the morphology of the wing and photoreceptor cell in Drosophila, centrosome duplication and cell death signaling in mammals. In the previous study, we found MOB2 protein can induce the neurite formation in mouse N2A cell line and affect the migration of human hepatocarcinoma cell line (Mahluva cell). In this study, we used LS-SCA5 tissue microassay to evaluate the expression of hMOB2. Using immunohistochemistry stain, hMOB2 was generally expressed in varies type of tissues with different extent. Besides, we found the expression of hMOB2 is significantly increased in malignant tissues than normal tissues. This indicates hMOB2 may have a role in cancer development and progression. We further evaluated the function of hMOB2 by an in vitro assay using the colonal cancer cells (HT29 cell). We found that hMOB2 expressed in the cytoplasm of HT29 cells. When the expression of hMOB2 in HT29 cells was knocked down by shRNAi, cell proliferation and cell migration were both decreased significantly. Thus, hMOB2 protein had a role in cell proliferation and cell migration. All together, hMOB2 is a potential candidate for future study in cancer development and progression.