A green synthesis and characterization of gold nanostructures using biopolymers and their application in catalysis and biocompatibility

• Main Author: Emmanuel Naveen Raj Lawrence
• Other Authors: Shih, Yang-Chia
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/88esv5

碩士 === 亞洲大學 === 生物科技學系碩士班 === 101 === Nanotechnology is an emerging interdisciplinary science having vast applications in the field of life sciences, healthcare and industrial biotechnology. The development of green processes for the synthesis of metal nanostructures is evolving into an important branch of nanotechnology. Among various metal nanostructures especially gold nanostructures has emerged as attractive nanomaterials for various applications in electronics, photonics, catalysis, sensing, imaging and drug delivery due to their physical and chemical properties. This present study focused on a one pot green chemistry synthesis of gold nanostructures using thermal reduction by utilizing the interaction of chloroauric acid with biopolymers such as gellan gum and hyaluronic acid as reducing, shape directing and stabilizing agents. The optical properties and morphology of the synthesized gold nanostructures are characterized by UV-visible spectroscopy, field emission scanning electron microscopy (FESEM), high resolution transmission electron microscopy (HRTEM), fourier transform infra-red spectroscopy (FTIR) and X-ray diffraction (XRD). From the analyses of UV-vis spectra, FESEM, HRTEM, FTIR and XRD patterns revealed the formation of gold nanostructures with crystalline nature and functional groups which are generated by manipulating key growth parameters such as concentration of biopolymers and gold salt. The catalytic activity of the synthesized gold nanostructures was investigated by monitoring photometrically the reduction of 4-nitrophenol by an excess of NaBH4 and which resulted in better catalytic activity. The invitro cytotoxicity effect of different concentration of gold nanostructures such as long nanowire, short nanowire, and spherical particles were investigated in primary human fibroblast cell (HSF) using MTT assay which resulted in non-cytotoxicity and highly biocompatible. This present study opens up a new possibility of generating variously shaped gold nanostructures through a simple and green approach which may be useful in the design of novel materials as well as paving the way for optical, structural properties and biomedical applications.