| Summary: | 碩士 === 東吳大學 === 微生物學系 === 101 === In Taiwan, cancer has been reelected to the ten leading causes of death for up to three decades. The death rate increased year by year. Surgical resection and chemotherapy are the major way to cure cancer. But surgical resection has its risks and also can not completely remove the cancer cells. And surgical resection may even result in the transfer of cancer cells. For these reasons, chemotherapy still the major research direction. The anti-microbial activity of antimicrobial peptides as antibiotic alternative medicine is a wide range of research. Some reports have shown that antimicrobial peptides may induce cancer cell death via apoptosis, necrosis and autophagy.
Herein, we test the lipopolysaccharide binding peptides with anti-microbial activity to observing it’s cell cytotoxicity and cell cycle effect in five types of cancer cells. Therefor, we try to assess this peptide's anti-cancer ability.
The results show that LPSBD0 and LPSBD2 has cytotoxicity to all kind of cancer cell in our research. But the different cancer cells treat with same peptide has different effection. For instance, liver cancer and lung cancer cells are more sensitive to this lipopolysaccharide-binding peptide. Moreover, these lipopolysaccharide-binding peptides will affect the cell cycle of all kinds of cancer cells. And this lipopolysaccharide-binding peptide cause cells to produce large amounts of debris. So, we consider that lipopolysaccharide-binding peptide has the ability to kill cancer cells. Use confocal microscopy to observe treated cells. Show that lipopolysaccharide-binding peptides, LPSBD0 and LPSBD2, are binding on the cell membrane. And different peptide treat with different cell has different affinity. In low concertration, these lipopolysaccharide-binding peptides can disrupt the cell membrane. So, we presume that lipopolysaccharide-binding peptides kill cells by destroy the membrane.
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