| Summary: | 碩士 === 實踐大學 === 食品營養與保健生技學系碩士班 === 101 === Gut microbiota is critical for the normal structural and functional development as well as optimal physiological metabolism functions in the intestine. In mammals, the Toll-like receptors 2 (TLR2) have evolved to recognize conserved products unique to microbial metabolism. This receptor plays a crucial role in detection of microbial infections. The gut microbiota possibly interacts with TLR2 to influence physiological metabolisms. In the present study, we analyzed of the effects of gut microbiota in the germ-free mice (GF), the specific pathogen free (SPF) mice and the TLR2 knockout mice (TLR2(-/-)) by proteomic analysis. We used one dimensional gel and nano-LC-MS/MS to detect the liver proteins in the GF, SPF and TLR2(-/-) mice to determine their physiological metabolism and energy usage. The results identified 24 differentially expressed proteins in GF mice inculding 8 up-regulated protins and 16 down-regulated proteins (T-tast, p-value less than 0.05). In TLR2(-/-) mice, 37 differentially expressed proteins were identified including 19 ur-regulated protins and 18 down-regulated proteins. Furthermore, Ingenuity Pathway Analysis (IPA) indicated that intestinal gut flora influence the physiological metabolism in synthesis of glycogen and fructose, lipid metabolism of β-oxidation and amino acid degradation in the GF mice. While in TLR2(-/-) mice, physiological metabolism is affected in glycolysis, pentose phosphate pathway and β-oxidation. Our results suggest that the physiological metabolism and energy usage are altered in GF mice compared to the TLR2(-/-) mice. It is likely that the GF mice is lack of the gut bacteria to regulate the change of the physiological metabolism and energy usage. However, the TLR2(-/-) mice have the TLR2 gene knocked out, the gut bacteria could not interacts with TLR2 to execute the functions of physiological metabolism and energy usage.
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