Proteomic Analysis of Aging-Related Biomarkers in Mouse Serum

• Main Authors: Hou, Hsiang-Lin, 侯湘伶
• Other Authors: Lin, Wen-yuan
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/90634557435628275145

碩士 === 實踐大學 === 食品營養與保健生技學系碩士班 === 101 === Due to the growing populations of aging, development of biological markers for prediction of aging-related chronic diseases and prognosis tracking becomes an important issue for current biomedical research. The normal aging process is inevitable and universal. It is characterized by a general decline in physiological functions with accumulation of multiple deleterious effects. Based on the free radical theories related to the aging processes, the major source of the oxidative lesions comes from generation and accumulation of oxidants in the mitochondria within cells, becoming one of the factors leading to aging and death. In this study, we applied proteomic strategies to detect and compare serum proteins between 7 months (middle-aged) and 24-month-old (aged) mice in order to explore and isolate biomarkers of normal aging processes. The results showed that thirteen proteins were detected at different levels in expression significantly. Among these proteins, five are related to acute reactions and three are involved in the immune responses. Overall, the inflammation responses appeared to go up during aging. Further post-translational modification analysis on serum albumin in mice indicated there are four different kinds of modification related to oxidative stress including Nitration, Carbonylation, Homocysteine and 4-Hydroxy-2-nonenal; the modification ratio was significantly increased in the aged mice. Totally, all the results of this study will generate specific serum aging-related biomarkers to survey aging-related chronic diseases and prognosis tracking following treatment.