Effect of retinoic acid, Galectin-1 and gold nanoparticles on neurite outgrowth of N2A Cells

• Main Authors: Chang, Yi-Jye, 張怡捷
• Other Authors: Chiang, Ming-Chang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/35264148003036818243

碩士 === 中國文化大學 === 生物科技研究所 === 101 === Many studies suggest that Retinoic acid (RA) plays an important role in neural development. RA is a product of Vitamine A metabolism, which controls physiological processes including development, nervous system function, cell proliferation and differentiation, through the two nuclear receptors, retinoic acid receptors (RAR) and retinoid X receptors (RXR). Galectin-1 (GAL-1) is one of galectin family, GAL-1 is a β-galactosyl binding lectin, which can bind with β-galactosides. GAL-1 can promote cell growth, differentiation, adhesion, and also participates in regulating cell proliferation. Gold nanoparticles (AuNPs) a novel agent, has good biocompatibility and has been used in biomedical researches. Investigations suggest AuNPs can regulate cell function, promote cell differentiation. In this study, N2A cells (neuroblastoma cells), were divided into control, RA (10 M), GAL-1 (200 ng/ml) and AuNPs (5 ppm), respectively. Experiments were divided into two parts. First, we use microscope and Micrometrics SE Premium to observe neurite outgrowth, by taking photograph and meansuring length of axon. Secondly, this study used 3-(4,5-dimetnyl-thiazol-2-yl)2, 5-diphenyl tetrazolium bromide (MTT), real-time quantitative polymerase chain reaction (QPCR) and mitochondrial function mass to analysis cell viability, mitochondrial function and gene expression. Our results show that RA, GAL-1 and AuNPs all promote neurite outgrowth of N2A cells and significantly increase the neurite length. The results show that N2A cell neurite outgrowth is better than control group with RA, GAL-1 and AuNPs treat. Neurite outgrowth can be used as the index of neuronal differentiation. Our results show that RA, GAL-1 and AuNPs may also have neurite repair potential.