Develop and Research of Aquatic Animal Waste Apply to Orthopedic Materials - Taiwan Tilapia as the Example

• Main Authors: Yao, Ci-Chih, 姚其治
• Other Authors: Lin, Shang-Ming
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/cq229z

碩士 === 亞東技術學院 === 應用科技研究所 === 102 === This study focuses on waste of scales from Taiwan Tilapia to research and development, rather than merely ordinary extraction method of Collagen and Hydroxyapatite. Whole pieces of scale are treated by the purifying process, and proceeded analysis of surface structure, nuclear dyeing, moisture content, contact angle and thermal properties. There are two different structures of rough and smooth on scale surface, most of rough structures show wavy arrangement, other districts are irregular honeycomb. By the purifying process, scale can be removed impurities and cells, it displays about 46℃ absorption peak of Type I collagen after purifying process, and inorganic at about 45wt%. The material preparation can be divided into collagen powder and porous scaffold, to beginning with purifying scales are smashed by Air Separated Grinding Machine into collagen powders, and particle size is classified according to below 25μm, 25～45μm, 45～100μm and above 100μm by sieve. SEM analysis shows not smooth but complex lumpy surface on particle. Lastly base on one kind of concentration of Collagen powder from scale to compound various concentrations of Gelatin, besides there are two cross-linking agents(GA、BDDGE) in reaction for gelling, and through lyophilization turn into porous scaffold, then proceed tests of porosity, cytotoxicity, moisture content and mechanical properties, it displays maximum is 150μm, and minimum is about 50μm of diameter of hole. The BDDGE group presents cell viability over 70% conforms to ISO10993, and the GA group with cytotoxicity as agent concentration increases.Top of Young’s Modulus is 63.65MPa, Yield Strength attains to 2.39Mpa, we expect the study which can apply to fill non-loaded region of defected bone and dental filler.