Detection of microorganisms using self-assembly of functionalized microspheres

• Main Authors: Hui-Hsien Lin, 林慧賢
• Other Authors: James R. Carey
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/24361825505677795333

碩士 === 國立高雄大學 === 應用化學系碩士班 === 101 === In the recent decades, the improvement of the diagnostic detection method is focusing on raising the sensitivity, enhancing the detection limit, and speeding up the diagnosis process. The purpose of my research is to amplify the initial signals from the antibody-antigen complexes. The signal enhanced media are the streptavidin modified microspheres and the biotin coated microspheres. The hypothesis of our strategy is if the analytes are detected by the antibody coated surface, polymerization of the microspheres is triggered and can amplify the initial signals and lead to visual detection. The polymerization is achieved by the streptavidin-biotin interaction, which is known to be one of the strongest non-covalent bonds in nature. The visual detection of the targets is accomplished by the self-assembly of functionalized microspheres which can be observed by naked eye or a commercial microscope. This simple detection method may be used to identify infectious microorganisms in few steps.