Fabrication of Fe3O4@SiO2@anti-ALB/CdTe Core-shell Nanoparticles for Optical Immunosorbent Assay with Magnetolithography

• Main Authors: Pao-yu Liao, 廖寶玉
• Other Authors: Jem-kun Chen
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/06890311810624921585

碩士 === 國立臺灣科技大學 === 材料科學與工程系 === 101 === In this study, we fabricated superparamagnetism nanoparticles and core-shell structures comprising Fe3O4 and SiO2 as the core and shell components, respectively. After surface modified with NH2 group(MNPs), Protein G was bound to the surface of MNPs for increasing the orientation of anti-ALB. Then, anti-ALB and CdTe were bound to MNP@Protein G and we detected ALB by the shelter effect of CdTe. In addition, we patterned composite nanoparticles by Ni-mask with magnetolithography for immunosorbent assay. Superconducting Quantum Interference Device Magnetometer (SQUID) and Transmission Electronmicro-scope(TEM) revealed superparamagnetism and the core/shell structure. Then we investigated the orientation of anti-ALB with the sheltered efficiency and the ability of binding antigen by Spectrofluorometer(FP) and Enzyme-linked immunosorbent assay (ELISA). We also demonstrated the amounts of ALB and the sheltered efficiency were achieved to 2.2614μg/mg and raised to 59.40%, respectively. We could observe the difference of the fluorescent intensity significantly. Finally, the Ni-masks were successful fabricated with the line-width (1μm and 3μm) and hole-width (500nm). Then, we demonstrated we used the Ni-mask of line-width 3μm to show the smallest scale and complete pattern for optical immunosorbent assay by Optical Microscope(OM) and Confocal Spectral Microscope Imaging System(CLSM).