| Summary: | 碩士 === 國立臺灣科技大學 === 材料科學與工程系 === 101 === In this study, we fabricated superparamagnetism nanoparticles and core-shell structures comprising Fe3O4 and SiO2 as the core and shell components, respectively. After surface modified with NH2 group(MNPs), Protein G was bound to the surface of MNPs for increasing the orientation of anti-ALB. Then, anti-ALB and CdTe were bound to MNP@Protein G and we detected ALB by the shelter effect of CdTe. In addition, we patterned composite nanoparticles by Ni-mask with magnetolithography for immunosorbent assay.
Superconducting Quantum Interference Device Magnetometer (SQUID) and Transmission Electronmicro-scope(TEM) revealed superparamagnetism and the core/shell structure. Then we investigated the orientation of anti-ALB with the sheltered efficiency and the ability of binding antigen by Spectrofluorometer(FP) and Enzyme-linked immunosorbent assay (ELISA). We also demonstrated the amounts of ALB and the sheltered efficiency were achieved to 2.2614μg/mg and raised to 59.40%, respectively. We could observe the difference of the fluorescent intensity significantly.
Finally, the Ni-masks were successful fabricated with the line-width (1μm and 3μm) and hole-width (500nm). Then, we demonstrated we used the Ni-mask of line-width 3μm to show the smallest scale and complete pattern for optical immunosorbent assay by Optical Microscope(OM) and Confocal Spectral Microscope Imaging System(CLSM).
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