A Mechanism of High Throughput Reagent Distribution for Large-Scale Disease Detection

• Main Authors: Ming-Han Wu, 吳明翰
• Other Authors: Pin-Chuan Chen
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/26587722367114236896

碩士 === 國立臺灣科技大學 === 機械工程系 === 101 === Rapid and uniform reagent distribution is critical to the performance of a high-throughput microfluidic system, and the geometry design of the microchannel dominations the accuracy and uniformity of the split droplet distribution. This study focuses on the uniform fission of a single liquid droplet in a T-junction microchannel and the aim is to understand the impact of the geometry design to the uniformity of the split droplets. A gas-liquid modeling was realized in the transient numerical simulation to investigate the pressure distribution and the flowing velocities inside the droplet during the splitting process, which leads to a modified design of the T-junction that minimizes the velocity difference inside the droplet and results in a more smooth splitting process. The polymer microfluidic devices were manufactured with micromilling and thermal bonding for droplet distribution experiments. In the multiple experiments, a 2 μl reagent was loaded into the microfluidic device and an uniform pneumatic pressure was applied to push the droplet downward to the T-junction for splitting. The experiment results reveal that a modified T-junction can achieve better uniformity, higher reliability, and less reagent loss in a modified T-junction during the droplet splitting process, which can be applied in designing a high throughput microfluids device for large-scale experiment.