| Summary: | 碩士 === 國立臺灣科技大學 === 醫學工程研究所 === 101 === Hearing loss is the most common sensory impairment in humans, and the causes are dysfunction, injury, or death of the hair cell. Recently, gene therapy has been reported to insert genes encoding functional proteins that can preserve, protect, or regenerate hair cells in the inner ear. In our previous study, a basis for microbubbles (MBs) ultrasound (US)-mediated techniques with therapeutic medication delivery to the inner ear for future application in humans has been demonstrated. In this study, cationic liposomes were as the delivery vectors and the feasibility of albumin MBs US-mediated techniques in different sizes MBs with gene delivery to the inner cells has been investigated. According to the results, the MBs size was in direct proportion to the increase of the concentration of albumin or dextrose, respectively. With the same US energy, the transfection of albumin MBs with 0.66, 1.32, 2.83 μm in diameter increase 30.6% , 54.1% and 84.7% . The transfection of dextrose albumin MBs with 1.39, 2.12, 3.47μm in diameter increase 15.9%, 34.3% and 82.7%. To increase MB size and control property of elasticity produce desired levels of US mediated gene transfection. The MBs sizes in different condition have been determined and their oscillation behavior is important for effective gene transfection using US. The optimal MBs size for gene transfection in the inner ear cells with US has been evaluated.
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