Application of Decellularized Adipose Tissue/Silk Fibroin Matrix and Microspheres in Adipose Tissue Engineering

• Main Authors: Chen-Han Tseng, 曾琛涵
• Other Authors: Yi-You Huang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/47831236297898866830

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 101 === The soft tissue defects caused by congenital malformation, trauma, tumor removal and other various reasons affect the patient''s psychology and interpersonal relationship, so it has been gaining popularity for Plastic and Reconstructive Surgery. Due to advances in medicine, the emphasis on the requirements of the quality of medical care and physical appearance is increasing, and it also increases the demand for medical cosmetic. However, current challenge of adipose tissue engineering failed to maintain volume of adipose after transplantation, so it is important to find the soft fillers that have both functionality and aesthetics. In the study, we used decellularized porcine adipose matrix and silk fibroin as composite materials for scaffolds. The loss of the ECM content mixed in the scaffolds was slowed down by cross-linking. Eighty percentage collagen and thirty percentage GAG contents were retained after removing most cells and lipids from porcine adipose tissue. These bioactive contents have been proved to induce cell differentiation, and hydrolyzed silk fibroin also has the ability to promote cell proliferation. In addition, both of these materials have high biocompatibility, and they can increase the overall mechanical properties after blending. In vitro experiments, we observed that 3T3-L1 and adipose stem cells attached to the composite scaffolds successfully, and their GAG content increased 30 % to 45 % after culture several days, and there was remarkable difference between the composite scaffolds and the silk fibroin scaffolds. In vivo experiments, we observed that adipose matrix-silk fibroin composite microspheres were more effective to promote adipose stem cells proliferation and differentiation than silk fibroin microspheres. There are great potentials for the application of these hybrid materials in dermal fillers and soft tissue regeneration.