Electric Field-induced Lipid Raft Polarization Guide Fibroblast Directional Migration

• Main Authors: Bo-Jiang Lin, 林栢江
• Other Authors: Pen-Hsiu Grace Chao
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/16001872841993951199

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 101 === Galvanotaxis is a phenomenon in which microorganisms migrate in response with the electric current. Most studies indicate that the redistribution of plasma membrane proteins guides cell directional motility. The previous study showed that α2β1 integrin polarizes with AC and DC electric fields. This study shows when fibroblasts are stimulated by an electric field, lipid rafts polarize and the polarization coincides with asymmetrically-distributing α2β1 integrins. Disruption of lipid rafts inhibits EF-induced directional migration. The caveolin-1 knockdown inhibits cell directional motility and RhoA polarization. The results indicate that lipid raft is a mechanosensor to EF stimulation and lipid raft polarization lead to integrin and caveolin-dependent directional migration.