Development of orange-spotted grouper iridovirus (OSGIV) myristylated membrane protein subunit oral vaccine preparation and microencapsulation

• Main Authors: Chang-Yu Tsai, 蔡昌佑
• Other Authors: 陳秀男
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/pe7c2q

碩士 === 國立臺灣大學 === 漁業科學研究所 === 101 === The vacccine has to be protected against gastric acid and digestive system. My thesis uses microencapsulation technique to encapsulate OSGIV (orange spotted grouper iridovirus, OSGIV) subunit vaccine from digestive system. Microencapsulation material is sodium alginate, its properties are low toxic, low antigenic and low cost. Using sodium alginate as microencapsulation material is called microcapsule. It is acid-stable ,but it will be collapsed when pH value arises in base environment. Therefore, microcapsule can make vaccine safely passing through stomach and be released in postgut. In my thesis, microcapsule loading rate is 7.74%±1.23%，encapsulation efficiency is 50.46%±8.03%. Mix microcapsule and grouper artificial feed and keep feeding Epinephelus lanceolatus for four weeks, I find out that the groups of fed oral vaccine’s specific antibody in gut arise faster then serum. The results are the group of fed low dosage oral vaccine’s specific antibody can keep eight weeks and the group of fed high dosage oral vaccine’s specific antibody can keep ten weeks. Based on my thesis, the microcapsule of oral vaccine can induce immune response to produce specific antibody successfully.