Functional Studies of FIN219 and HFR1 Regulatory Relationship in the Integration of Shade Avoidance Syndrome and Jasmonate Signaling in Arabidopsis

碩士 === 國立臺灣大學 === 植物科學研究所 === 101 === Light is one of the most effective environment factors that regulate plant growth and development. Different light sources have their own effects on seedlings. Shade-avoidance syndrome (SAS) is the phenotype that occurs at low red to far-red ratio, which will re...

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Main Authors: Hui-Chen Kuo, 郭蕙禎
Other Authors: Hsu-Liang Hsieh
Format: Others
Language:en_US
Published: 2013
Online Access:http://ndltd.ncl.edu.tw/handle/58423515428105238788
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spelling ndltd-TW-101NTU053660072016-03-16T04:15:06Z http://ndltd.ncl.edu.tw/handle/58423515428105238788 Functional Studies of FIN219 and HFR1 Regulatory Relationship in the Integration of Shade Avoidance Syndrome and Jasmonate Signaling in Arabidopsis 在阿拉伯芥避蔭效應與茉莉酸訊息傳遞中FIN219與HFR1調控關係的功能性研究 Hui-Chen Kuo 郭蕙禎 碩士 國立臺灣大學 植物科學研究所 101 Light is one of the most effective environment factors that regulate plant growth and development. Different light sources have their own effects on seedlings. Shade-avoidance syndrome (SAS) is the phenotype that occurs at low red to far-red ratio, which will reduce the yield of crops. Although it has been noticed that jasmonates are involved in SAS, the molecular regulation between jasmonates and SAS are still unknown. LONG HYPOCOTYL IN FAR-RED 1 (HFR1) is known as a negative regulator of SAS, and FAR-RED INSENSITIVE 219 (FIN219) is reported to be a positive regulator in JA signaling. Both HFR1 and FIN219 participate in phyA signaling and they are required for SAS. Besides, COP1 has been reported to interact with FIN219 and HFR1, respectively. It is interesting to find the relationship between HFR1 and FIN219, and their regulation during SAS and JA signaling. Here, we found that fin219hfr1 double mutant is insensitive to JA and has even longer hypocotyl phenotype than single mutants in shading treatment. Gene expression analyzer indicated that HFR1 also participates in JA signaling and positively regulates FIN219 expression, whereas FIN219 participates in SAS signaling and negatively regulates HFR1 expression. Protein expression studies showed the same regulatory patterns of FIN219 and HFR1. Moreover, co-immunoprecipitation studies indicated that FIN219 and HFR1 did not physically interact with each other, which implies that other regulators may be involved in this regulation mechanism. Taken together, our data indicate that FIN219 and HFR1 play vital roles in regulating seedling development in response to JA and shading conditions. Hsu-Liang Hsieh 謝旭亮 2013 學位論文 ; thesis 72 en_US
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language en_US
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description 碩士 === 國立臺灣大學 === 植物科學研究所 === 101 === Light is one of the most effective environment factors that regulate plant growth and development. Different light sources have their own effects on seedlings. Shade-avoidance syndrome (SAS) is the phenotype that occurs at low red to far-red ratio, which will reduce the yield of crops. Although it has been noticed that jasmonates are involved in SAS, the molecular regulation between jasmonates and SAS are still unknown. LONG HYPOCOTYL IN FAR-RED 1 (HFR1) is known as a negative regulator of SAS, and FAR-RED INSENSITIVE 219 (FIN219) is reported to be a positive regulator in JA signaling. Both HFR1 and FIN219 participate in phyA signaling and they are required for SAS. Besides, COP1 has been reported to interact with FIN219 and HFR1, respectively. It is interesting to find the relationship between HFR1 and FIN219, and their regulation during SAS and JA signaling. Here, we found that fin219hfr1 double mutant is insensitive to JA and has even longer hypocotyl phenotype than single mutants in shading treatment. Gene expression analyzer indicated that HFR1 also participates in JA signaling and positively regulates FIN219 expression, whereas FIN219 participates in SAS signaling and negatively regulates HFR1 expression. Protein expression studies showed the same regulatory patterns of FIN219 and HFR1. Moreover, co-immunoprecipitation studies indicated that FIN219 and HFR1 did not physically interact with each other, which implies that other regulators may be involved in this regulation mechanism. Taken together, our data indicate that FIN219 and HFR1 play vital roles in regulating seedling development in response to JA and shading conditions.
author2 Hsu-Liang Hsieh
author_facet Hsu-Liang Hsieh
Hui-Chen Kuo
郭蕙禎
author Hui-Chen Kuo
郭蕙禎
spellingShingle Hui-Chen Kuo
郭蕙禎
Functional Studies of FIN219 and HFR1 Regulatory Relationship in the Integration of Shade Avoidance Syndrome and Jasmonate Signaling in Arabidopsis
author_sort Hui-Chen Kuo
title Functional Studies of FIN219 and HFR1 Regulatory Relationship in the Integration of Shade Avoidance Syndrome and Jasmonate Signaling in Arabidopsis
title_short Functional Studies of FIN219 and HFR1 Regulatory Relationship in the Integration of Shade Avoidance Syndrome and Jasmonate Signaling in Arabidopsis
title_full Functional Studies of FIN219 and HFR1 Regulatory Relationship in the Integration of Shade Avoidance Syndrome and Jasmonate Signaling in Arabidopsis
title_fullStr Functional Studies of FIN219 and HFR1 Regulatory Relationship in the Integration of Shade Avoidance Syndrome and Jasmonate Signaling in Arabidopsis
title_full_unstemmed Functional Studies of FIN219 and HFR1 Regulatory Relationship in the Integration of Shade Avoidance Syndrome and Jasmonate Signaling in Arabidopsis
title_sort functional studies of fin219 and hfr1 regulatory relationship in the integration of shade avoidance syndrome and jasmonate signaling in arabidopsis
publishDate 2013
url http://ndltd.ncl.edu.tw/handle/58423515428105238788
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