| Summary: | 碩士 === 國立臺灣大學 === 植物科學研究所 === 101 === Light is one of the most effective environment factors that regulate plant growth and development. Different light sources have their own effects on seedlings. Shade-avoidance syndrome (SAS) is the phenotype that occurs at low red to far-red ratio, which will reduce the yield of crops. Although it has been noticed that jasmonates are involved in SAS, the molecular regulation between jasmonates and SAS are still unknown. LONG HYPOCOTYL IN FAR-RED 1 (HFR1) is known as a negative regulator of SAS, and FAR-RED INSENSITIVE 219 (FIN219) is reported to be a positive regulator in JA signaling. Both HFR1 and FIN219 participate in phyA signaling and they are required for SAS. Besides, COP1 has been reported to interact with FIN219 and HFR1, respectively. It is interesting to find the relationship between HFR1 and FIN219, and their regulation during SAS and JA signaling. Here, we found that fin219hfr1 double mutant is insensitive to JA and has even longer hypocotyl phenotype than single mutants in shading treatment. Gene expression analyzer indicated that HFR1 also participates in JA signaling and positively regulates FIN219 expression, whereas FIN219 participates in SAS signaling and negatively regulates HFR1 expression. Protein expression studies showed the same regulatory patterns of FIN219 and HFR1. Moreover, co-immunoprecipitation studies indicated that FIN219 and HFR1 did not physically interact with each other, which implies that other regulators may be involved in this regulation mechanism. Taken together, our data indicate that FIN219 and HFR1 play vital roles in regulating seedling development in response to JA and shading conditions.
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