| Summary: | 碩士 === 國立臺灣大學 === 食品科技研究所 === 101 === Diabetes mellitus (DM) is the 5th leading cause of death in Taiwan, and the prevalence is still growing. More than 95 % among all the cases belong to type 2 diabetes and characterized as insulin resistance that resulted in hyperglycemia. Phenolic acids are widely distributed in vegetables and fruits. Although some studies have shown the effect of phenolic acids on anti-hyperglycemia, there has been no systematic study on DM yet. In this study, the insulin resistant mouse hepatocytes FL83B cell model was established and used as the screen platform. The cell viability test showed that the critical safe dosage of 9 phenolic acids is 12.5 μM. The result from glucose uptake test showed that vanillic acid exhibits the highest increment in glucose uptake in insulin resistant cells among tested samples. Animal model was then performed to assess the effect of vanillic acid in high fat diet-fed rats. Male Sprague-Dawley rats are randomly divided into two groups, including a control group (fed with chow diet containing 14 % kcal from fat), and a high-fat diet group (fed with high-fat diet containing 60 % kcal from fat) for the development of hyperglycemia. Furthermore, the high-fat diet group were further divided into three sub-groups, including the high-fat diet sub-group, positive control sub-group (orally administrated pioglitazone, 30 mg/kg b.w.) and treatment sub-group (orally administrated vanillic acid, 30 mg/kg b.w.). The results showed that vanillic acid significantly alleviated high-fat diet induced syndrome, including hyperglycemia, hyperlipidemia, hyperleptinemia and insulin resistance. We thus demonstrated the potential of vanillic acid in the development of health foods or dietary supplements for the prevention of hyperglycemia in prediabetes.
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