| Summary: | 碩士 === 國立臺灣大學 === 生理學研究所 === 101 === Breast cancer is associated with high-fat intake. About two thirds of breast cancer in women are estrogen-dependent that contain estrogen receptor . It has been found that not only estrogen, the estrogen metabolites can also bind with estrogen receptor for the regulation of breast cancer development. We aimed to examine the effect of high fat diet on estrogen metabolism during reproductive age and pregnancy in rats. The urine was collected during 5-day estrus cycle in adult rats fed chow or high n-6 sunflower oil diets for 80 days, or collected at gestation day 10 or day 18 in pregnant rats fed the chow diet, high n-9 safflower oil, high n-6 sunflower oil without or with fish oil supplementation, low fat or high fat diets without or with fish oil supplementation. The 12 estrogen metabolites in urine was measured by HPLC with 8-channel electrochemical detector. We found that total estrogen metabolites, 16 -hydroxyestrone, 2-hydroxyestrone and estriol were increased in rat urine fed the high n-6 sunflower oil diet during reproductive age and in pregnancy compared with that fed chow or low fat diets. The methylation of 4-hydroxyestrogen and 2-hydroxyestrogen was decreased in rat urine fed the high n-6 sunflower oil or high n-9 safflower oil diets during reproductive age and in pregnancy compared with that
fed chow or low fat diets. And this decreased effect of methylation of 2-hydroxyestrogen by high n-6 fat diet was increased back by fish oil supplementation during pregnancy. We proposed that carcinogen 16 -hydroxyestrone is increased and the anti-carcinogen 2-methoxyestrone is decreased in female rats fed high fat diet.
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