Association Study of Aspirin Resistance with Platelet Surface Markers, Monocyte Surface Markers and Microparticles in Diabetes

• Main Authors: Wei-Che Huang, 黃偉哲
• Other Authors: Fu-Tien Chiang
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/71250958985042592953

碩士 === 國立臺灣大學 === 生理學研究所 === 101 === Introduction: Aspirin is integral in the primary and secondary prevention of coronary artery disease and acute coronary syndrome. However, the effect of aspirin on antiplatelet is not very well in diabetes called aspirin resistance (AR). In this study, we investigate whether AR in diabetes is associated with platelet surface receptors, monocyte surface receptors and microparticle. Materials and methods: A total of 70 patients with cardiovascular disease were enrolled. Twenty-four patients had diabetes and six of them had AR. Forty-six patients were diabetes and eight of them were AR. Blood samples were obtained twice for this study, first at baseline (pre-ASA), and second was after at least two weeks following the post-ASA. All subjects received one 100 mg aspirin. (Bokey EM /cap 100 mg or Tapal/tab 100 mg ). We use the PFA-100 to evaluate platelet aggregation and flow cytometry to evaluate CD62p (P-selectin), PAC-1, CD31, CD42a, CD14 and CD62E. Results: The PAC-1 expression was enhanced in diabetes with AR in post-aspirin than in pre-aspirin (0.12±0.05, 0.03±0.04, p<0.05). The CD62E counts reduced in post-aspirin compared to pre-aspirin (171.30±79.00, 237.73±123.82, p<0.05). Conclusion: AR in diabetes is associated with high PAC-1 expression. Although the effect of aspirin on antiplatelet is not very well in diabetes, it still is a vasoprotective agent for CVD.