Logic Gates of Silver Nanoclusters Controlled by Adenosine 5’-Triphosphate and pH

• Main Authors: Jennifer Daneen Lee, 李丹寧
• Other Authors: 張煥宗
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/41837339193914591329

碩士 === 國立臺灣大學 === 化學研究所 === 101 === Oligonucleotide templated Ag nanoclusters (OND–Ag NCs) have been prepared and used for construction of NAND, NOR, and IMPLICATION (IMP) logic gates, as well as for the detection of adenosine-5’ triphosphate (ATP). The OND–Ag NCs are prepared from AgNO3 using an OND with a sequence of 5’-TAACCCCTAACCCCT-3’ as a template and NaBH4 as a reducing agent. The fluorescence intensity and emission wavelength of OND–Ag NCs are dependent on the pH value and ATP concentrations. At pH 3.0 and 11.0, ATP shows greater effects on fluorescence of the OND–Ag NCs. Upon increasing ATP concentration from 10 to 50 μM, the emission wavelength of OND–Ag NCs at pH 3.0 shifts from 525 to 585 nm. At pH 11.0, the fluorescence intensity (510 nm) increases upon increasing ATP concentration. Through control of two inputs—[H+] and ATP concentrations—, and use of fluorescence intensity (IF510) and ratio (IF510/IF585) of OND–Ag NCs at pH 3.0 as outputs, NAND and NOR logic gates have been developed, respectively. Using [OH−] and ATP concentrations as inputs and IF510 of OND–Ag NCs at pH 11.0 as an output, an IMP gate has been demonstrated. The OND–Ag NCs provide linearity of IF525/IF585 ratios against ATP concentration over the range of 0.1–10 μM, which allow determination of ATP concentrations in cell lysate samples.