The effects of compositions of Phosphate Bioceramics on osteoinduction

• Main Authors: Han-Sheng Chen, 陳翰生
• Other Authors: Wei-Bor Tsai
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/71962306937613228153

碩士 === 國立臺灣大學 === 化學工程學研究所 === 101 === Bone grafts are aimed to stimulate osteogenesis in bone defects, and restore the structure and function of bone tissue. Since the atomic composition and mechanical properties of hydroxyapatite (HA) resemble the inorganic portion of bone tissue, HA becomes a popular material for synthetic bone grafts. Clinical evidence reveals that HA bioceramics process excellent biocompatibility and the property of osteoconduction. Although it is generally thought that HA lacks the osteoinduction property, some animal implant experiments reveal that osteoinduction may happen in the surroundings of HA in the bone defects. Nevertheless, such phenomenon is usually ignored in the studies of bone grafts. We suggest that it might be due to the adsorption of BMPs to HA from bone marrow or the stimulation in the secretion of BMPs from mesenchymal stem cells when they are cultured on HA. The composition or structure of calcium phosphate ceramic will affect its osteoinductive effect. First of all we would like to explore whether calcium phosphate ceramics have osteoinduction in the bone marrow cavity environment. We used different composed of calcium phosphate ceramics to observe the 3A6 mesenchymal stem cells growth and differentiation status. And we detect the surface structure and physical properties of calcium phosphate ceramics to infer the effect of ceramic structure and composition on cell growth and differentiation, and thus we identify the most suitable composed of calcium phosphate ceramics for the bone graft material. In addition, we also observed whether the ceramic added BMP-2 can promote cell differentiation. Ceramic which adsorbed BMP-2 well is more easy to promote cell differentiation. In this study, we used five different composition of calcium phosphate ceramics to conduct experiments, and we analyzed the effect of various material properties on cell growth and differentiation. We verified that the addition of phosphate does make hydroxyapatite osteoinductive, and has also found that the higher the percentage of phosphate, the effect of osteoinduction is better. With a variety of experimental results, we identify the most suitable composition for bone filling. This formula can be used to product new bone filling material in future.