Immobilization of 3D culture human adipose-derived stem cells within RGD modified alginate microspheres

• Main Authors: Chi-Hui Huang, 黃琦惠
• Other Authors: Jiashing Yu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/33091730671435573194

碩士 === 國立臺灣大學 === 化學工程學研究所 === 101 === Cell-based therapies offer an attractive approach for revascularization and regeneration of tissues. The aim of cell therapy is to replace, repair, or enhance the function of damaged tissues or organs. There are, however, two major drawbacks that need to be resolved before cellular allografts or xenografts can achieve clinical acceptance: control of immune rejection and control of implanted cell proliferation. Cell encapsulation by means of organic or biologically derived matrixes, such as alginate, appears to be an avenue by which these goals may be accomplished. Three dimensional biology promises to enable efficient differentiation of stem cells. We made use of a magnetically levitating 3D cell culture system, based on alginate microcarriers coated with a biomimetic surface, that supports the growth of primary and stem cells in 3D envirument. The present study was undertaken to explore the possible therapeutic efficacy of RGD (Arg-Gly-Asp) peptide-linked biomaterial in repairing the lower limb ischemia of a rodent model. In this work, hASCs immobilized in RGD-coupled alginate microspheres, with a binary composition of high and low molecular weight alginate, were investigated. The in vitro study of hASCs demonstrated that RGD-coupled alginate promoted cell adherence to the matrix. Since different extra-cellular matrix proteins (ECM) influence the cells behavior, immobilized within RGD-alginate microspheres showed metabolic activity, with an overall viability higher than 80%, growth and increase angiogenic growth factor expression. We believe that the use of adult stem cell therapy in ischemia patients allows for the development of new mature and stable capillaries in patients affected by the severe condition.