| Summary: | 碩士 === 國立臺灣大學 === 分子與細胞生物學研究所 === 101 === Cell growth, the driving force to cell proliferation, is the process of up-regulating macromolecular synthesis and thereby increasing biomass. In proliferating cells, the chromosome replication, the quantitative change of organelles, and all related regulating mechanisms must comply with fidelity. This is to ensure that every single copy cell is similar to the original cell. Cell containing these substances whose size control could also be affected by the inside and outside dynamical systems. To efficiently accumulate biomass, mitochondria switch the role of TCA cycle from producing energy to exporting much of the intermediates for biomass synthesis.
Saccharomyces cerevisiae can use its mother cell as template during cell growth, however mammalian cells do not coordinate their growing process in the same way. We suggest that mammalian cells gradually growing to twice the size may be associated with mitochondrial metabolism. In addition, the quantitative change of mitochondria in cell growth session should not only comply with the cell replication fidelity, but also abide by the principle of once-and-only-once replication.
The purpose of this study was to establish the correlation of mitochondrial numbers and cell size control by using Resveratrol as the main tool to promote mitochondrial biogenesis.
The results of this study reveal that: (1)Under the treatment with Resveratrol in HeLa cells, using the fluorescent dye NAO on flow cytometry to measure the mitochondrial number, and using forward scatter-width (FSC-W) to obtain the cell size, the cell size increases as the number of mitochondria increases. (2)After treating with Resveratrol for 24 hours and then removing it, we find that the number of mitochondria and cell size can return to the same degree of those in normal cells. (3)The number of mitochondria and cell size keep on increasing after the Resveratrol removed from the 48-hour treated object.
In this study, we establish the correlation between mitochondrial numbers and cell size control, and also discover mitochondria can be used as a template during the cell growth of mammalian cells. Moreover, we can use those cells whose Resveratrol influence has been removed after the 48-hour treatment as a whole new control group to develop other experiments.
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