| Summary: | 碩士 === 國立臺灣大學 === 分子與細胞生物學研究所 === 101 === Perfluorinated chemicals (PFCs), considered as endocrines disrupters and widely used in industries, are a group of stable man-made organic compounds. The potential health effects caused by PFCs exposures have been of great concerns. However, there is no toxicity-related study on PFUA, PFDoA, PFDS, and PFBA. Therefore, this study was aimed to study pharmacokinetics and preliminary toxicity test for PFUA, PFDoA, PFDS, and PFBA. Male SD rats (n=4) were treated with a single dose of PFUA by oral gavage at 0, 0.5 and 5 mg/kg. Subsequently, urine and serum samples were collected for 88 days and analyzed with a newly-developed on-line solid-phase extraction liquid chromatography tandem mass spectrometry (LC-MS/MS) method. These data were fit with pharmacokinetic models with the Win-Nonlin software. Concentration of serum estradiol, progesterone, testosterone, cholesterol, FSH and LH were measured by ELISA using commercial rat ELISA kits. PFUA and PFDoA were not detectable in urine. No significant differences were observed in PFUA and PFBA in pharmacokinetic parameters at 0.5 and 5 mg/kg to suggest linear pharmacokinetics. However, PFDoA presented non-linear pharmacokinetics. PFUA at the highest dose caused significant increase in serum estradiol levels but decrease in serum progesterone levels. PFDS at the highest dose caused significant decrease in serum testosterone levels. PFBA at the highest dose decrease in serum estradiol levels, implying that PFUA, PFDS and PFBA may affect steroidogenic hormone biosynthesis of male SD rats. These results first reveal that PFUA, PFDS and PFBA are environment endocrine disrupters and deserve further studies on its potential effects on human health.
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