Micropropagation of Jatropha curcas

• Main Authors: Yu-An Chen, 陳禹安
• Other Authors: 王亞男
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/13563154267425294668

碩士 === 國立臺灣大學 === 森林環境暨資源學研究所 === 101 === In this study, an efficient method for plant regeneration from a high-oil content strain(Liugui, Tianliao) of Jatropha curcas L. through organogenesis was developed. Leaves of field-grown J. curcas were treated with running water for 2 hours, soaked in 1/300(v/v) anticeptol solution and 70% ethanol solution in order, and treated with ultrasonic shaker for 30 seconds for each step, then soaked in 4% NaOCl solution with a drop of Tween 20 and shaking for 20 minutes and the leaves could get 86% non-contamination. After surface sterilization, mature embryos were placed in basal medium. The non-contamination rate was 98%. The most suitable medium for aseptic plantlets is MS medium and the length of plantlets could reach 3.99 cm in average. The best number of shoot buds(2.89) per explant and average length(1.70 cm) was observed when in vitro hypocotyl explants were placed in MS medium with 0.5 mg l-1 BA. The induced shoot buds were transferred to MS medium containing 0.5 mg l-1 BA for shoot elongation and 0.69 cm elongation was achieved after 4 weeks. The elongated shoots could be rooted on 1/2 MS with 1 mg l-1 IBA and the induction rate was 20%. The rooted plants could be outplanted in sterilized soil with 76.19% survival rate after 2 months.