Effect of the carbon nanotube/gelatin/polycaprolactone electrospinning nanofiberous matrix on behaviors of schwann cell

• Main Authors: Jiun-Chiang Huang, 黃俊強
• Other Authors: Fu-Yin Hsu
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/92463948754095593364

碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 101 === Electrospinning technque is a convenient method of fabricating nanofiber matrix. In this study, we fabricated carbon nanotube/gelatin/polycaprolactone nanofiberous matrix (PG-CNTs) by electrospinning technology. At the same electrospinning, the fiber diameters of PG-3%wt CNTs and PG-4%wt CNTs are 1.22±0.31 um and 0.50±0.10um, respectively. Hence we adjusted the electrospinning parameters to fabricate the similar fibrous diameter for the PG3C, PG4C and PG5C. Besides, we evaluated the adhesion and proliferation of Schwann cells seeded on various matrices. From the result of cell adhesion test, we found the CNTs would inhibit the Schwann cell attachment on PG-CNTs nanofiber matrices and membrane matrices. However, the Proliferation rate of Schwann cells on PG-CNTs nanofiber matrices is higher than on PG-CNTs membrane matrices. Moreover, there are no significant difference in cell proliferation among PG3C, PG4C and PG5C nanofiber matrices. From the western blot analysis, we found that the NRG protein expression were no significantly difference between PG-CNTs nanofiber matrices and PG-CNTs membrane matrices. But the levels of P0 protein expression is higher on the PG-CNTs nanofiber matrix than on the PG-CNTs membrane matrix. From above results, we found the main factor that influence the behavior of Schwann cell was topography, not composition of PG-CNTs matrices.