| Summary: | 碩士 === 國立臺灣海洋大學 === 機械與機電工程學系 === 101 === This thesis presents iron oxide-labeled cells in three-dimensional calcium alginate gel using magnetic fields by the microelectrode of adjusting and controlling immediately. Through manipulating the strength, orientation of the magnetic field and using crosslinking gradients in calcium alginate gel，we assembly of cell-laden microgels to generate natural tissue of Tissue Engineering research constructs with tunable microarchitecture and complexity in vitro and even directly in situ.Most of human organs consist of multiple types of cells organized in a complex pattern to meet specific functional needs .Such as those of the articular cartilage heart, lungs, and liver.
We culture BHK-21 with MnZn ferromagnetic particles between 0.1 and 10mg/mL that was added in Alginate gels，by controlling magnets generate natural tissue，on the other hand，certain species of particles can permit or even enhance tissue formation . According to the characteristic of hydrogenated amorphous silicon (a-Si:H), which impedance diminished after light illuminating. Not only used light to create real electrodes, but also combined optics setup with DMD projector to be the microelectrode of adjusting and controling immediately. Electrode patterns can be changed quickly and efficiently, and this method replaces the traditional microelectrode especially. Therefore it can control the positions and forms of calcium alginate gel formation what we want.
Taken together, this technique can be adapted for studying basic biological processes in vitro and applicable for the fabrication of complex tissue architectures required in tissue engineering.
Key words : tissue engineering, a-Si:H, digital micromirror device, the microelectrode of adjusting and controling immediately, Calcium alginate
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