A study of fast DNA detection with capacitance and impedance analysis on Lab-on-a-chip

• Main Authors: Dong-Ying Pai, 白東穎
• Other Authors: Chung-Chen Chang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/32004757095633422730

碩士 === 國立臺灣海洋大學 === 電機工程學系 === 101 === This study utilizes micro-electro-mechanical process technology to manufacture a microfluidic chip which consist of PDMS microfluidic on the upper layer and finger electrode on the lower layer. Using lithography technology and sputtering, a 100-nm platinum layer is deposited onto glass to be used as an electrode. Using MEMS technology, and using an SU-8 thick-film spin photoresist coating on the surface of a silicon wafer, a microfluidic master mold is produced. Using casting molds, PMDS microfluidic lines of a minimum width of 100 um are then transmitted, resulting in a PMDS microfluidic structure with a thickness of around 150 μm. Finally, the platinum layer and the PMDS structure are bonded together to form a hollow channel with a metallic layer, creating a microfluidic chip for DNA testing. Upon detecting biological matter, a DNA hybridization test is carried out using U-101 magnetic beads with probe ET996. The electric capacity and impedance are measured. Using DNA samples of the same concentration level, a hybrid and non-hybrid sample are compared for impedance and capacity levels to determine if the hybrid sample has indeed been hybridized. E.Coli DNA CCRC 10711 is used with U-101 magnetic beads to carry out hybridization in order to allow for the rapid detection and identification of pathogens.