Effects of Cistanche tubulosa Extract on Reproductive Function in Streptozotocin-Nicotinamide Induced Diabetic Male Rats

• Main Authors: Fan-Chi Ko, 柯汎其
• Other Authors: Zwe-Ling Kong
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/55864267329531854899

碩士 === 國立臺灣海洋大學 === 食品科學系 === 101 === Hypogonadism and oxidative stress occurs commonly in men with diabetes associated male infertility. KiSS1, GPR54, SIRT1 and SOCS-3 gene are related to metabolism of diabetes, that can regulate the reproductive axis by stimulation on GnRH neurons. Additionally, Cistanche tubulosa is the Chinese herbal medicine have traditionally been used for treatment of impotence, female sterility, and cold sensation in the loins and knees. The aim of this study was to investigate the capability of anti-inflammatory, anti-oxidative, and protective effects on male reproduction of Cistanche tubulosa extract against diabetic rats. In vitro, 10 µM echinacoside (major active material of Cistanche tubulosa extract) significantly inhibited reactive oxidative spices production in advanced glycation end products (AGEs) concentration (50 µg/ml) induced LC-540 cell and TM3 cell. The LC-540 cell has receptor for AGEs (RAGE), and 10 µM echinacoside could not only improve NF-κB activity, that is induced by the interaction of AGE and RAGE, but also significantly increase StAR, CYP11A1, CYP17A1 and HSD17β3 protein experission. In vivo, the model diabetic rat was induced by nicotinamide (230 mg/kg), streptozotocin (65 mg/kg) and 45% hight fat diet.Cistanche tubulosa extract was tested in three doses (80, 160 and 320 mg/kg, p.o daily) for 6 weeks. Besides, rosiglitazone (RSG) administration (0.571 mg/kg) as positive control. The results indicate that Cistanche tubulosa extract administration for 6 weeks improve hyperglycemia, insulin resistance, leptin resistance, and lipid peroxidation in diabetic rats. Besides, it has restored KiSS1, GPR54, SOCS-3, and SIRT1 mRNA expression in the hypothalamus, and recover sex hormone level. In conclusion, not only the antioxidant and anti-inflammatory activities but also steroidogenesis effect of Cistanche tubulosa extract might have contributed for its ability to decrease insulin resistance, leptin resistance and protect male reproduction.