| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 101 === Drug residue is an important issue in the food safety control. It is concerning about the toxicity, drug resistant and allergy. Husbandry workers use the antibiotics or anti-microbial for the disease prevention of animals. Gas phase chromatography mass spectrum (GC-MS) is a regulatory test method using in the antibiotics residue detection. It needs a central laboratory and cost consuming. A simple, rapid and sensitive detection method is necessary to detect the limit concentration of drug residue. In this study, we demonstrated novel approach of competitive assay which is combination of bead separation and signal producible liposome. The amine content antibiotics we have chosen to conjugate to the micro-bead. There are two strategies in this study: (1) used the antibiotic embedding liposome as competitor to compete the immunobead with analyte antibiotic molecular. The immunobead is a bead immobilized antibiotic specific antibody. In this strategy, there is one incubation: competition and signal binding in the same incubation. (2) Used the antibiotic bead combine protein G liposome technique strategy. In this strategy, we used the antibiotic bead as a competitor to compete the free specific antibody with analyte antibiotic molecule. Finally, we use the protein G liposome as the signal particular. In these two platforms, filter plate is used for the separation between the solid phase and liquid phase in the steps of washing, incubation and solution exchange, etc. In the result of the first strategy, which use immunobead combine with antibiotic embedding liposome, we cannot obtain any expected result. The dose-response correlation did not occur. But in the antibiotic bead combine protein G liposome technique strategy, we show the efficient of this platform using in the detections of ampicillin, Ceftiofur, Tetracycline and Gentamicin. In these four antibiotic detections the competitive correlations are significant. In the maximum residual limit concentration test, the result demonstrates the ability of the detection for the regulatory limit test of ampicillin, Ceftiofur and Gentamicin. The whole assay time was controlled within 2 hours. Concluding the study, this is an analysis platform which is low cost, rapid but sensitive approach for the drug residual limit test.
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