Development of Graphene Oxide Sheets for Surface Plasmon Resonance Biosensors

• Main Authors: Teng-Yi Huang, 黃騰毅
• Other Authors: Nan-Fu Chiu
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/68131920134228431119

碩士 === 國立臺灣師範大學 === 光電科技研究所 === 101 === The advantage of surface plasmon resonance (SPR) biosensors includes high sensitivity, label-free, and real-time detection. These advantage leading SPR biosensors to identified as one of the most important optical detection methods for biomolecules. The goal of this thesis is to develop graphene oxide (GO) sheets SPR biosensor that used GO as sensing layer for biomolecules detection. First, we used cystamine as linker layer, forming Cys film by self-assembled monolayer (SAM) technique. Then, GO solution was immobilized on the surface of Cys film through covalent attachment, forming GO sheets. The GO sheets SPR biosensor was used to detect the dynamic interactions of biomolecules and antibody-antigen. For experimental verification of this system, bovine serum albumin (BSA) and LAMP DNA products were used to demonstrate by GO sheets and conventional Au films. We also compared these two films by sensitivity, detection limits and kinetic analysis. The sensitivity of GO sheets that can be determined by this thesis is about 12-fold higher than that obtained with the sensor based on conventional Au films. The detection limits and the results of kinetic analysis of GO sheets were also better than conventional Au films. Most important of all, GO sheets have the ability for directly detect biomolecules, skipped the immunization interactions, leading to a low cost experiment. We anticipate that graphene oxide sheet SPR biosensors will not only enable for possible applications for biomedicine and diseases but also have wide spectrum of application in pharmaceutical industry, environmental monitoring, and agriculture in the near future.