Effects and mechanisms of a potential antimicrobial peptide on different cancer cells

• Main Authors: Guo-yang Huang, 黃國洋
• Other Authors: Yu-li Lo
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/20820209956795470113

碩士 === 國立臺南大學 === 生物科技學系碩士班 === 101 === Cancer has been known as one of the major causes of death around the world. A lot of manpower and research funding has been invested in the field of cancer medicine. There has been a small part of the cancer that has developed effective chemotherapy regimen. However, the side effects of chemotherapy and the arising multidrug resistance are main roadblocks for cancer treatment. Antimicrobial peptides represent a potential approach in the field of cancer medicine. Many antimicrobial peptides either purified or synthesized, have been confirmed to regulate the cell cycle and induce cancer cell apoptosis. The main purpose of this study is to use epirubicin combined with the antimicrobial peptide (E-1) in human cervical cancer and colorectal cancer cells. The cytotoxic effects and mechanisms of apoptosis induction were investigated. Real-time quantitative PCR, DNA fragmentation, and flow cytometric detection were performed. In addition, we also aim to explore the effect of this antimicrobial peptide on various cancer cell lines. The antimicrobial peptide E-1 has the potential to regulate the cell cycle and inhibit the proliferation of cancer cells, The results show that Antimicrobial peptides combined with the Epirubicin, Let reduce drug side effects including enhancing drug cytotoxic and lower doses of anticancer drugs. For the future of clinical medicine and cancer drugs have strong potential.