AC-ELECTROOSMOSIS-ASSISTED HIGH-DENSITY SIMUTANEOUS CONCENTRATION OF SERS NANOPARTICLES AND BIOMOLECULES FOR RAPID BIO-DETECTION

• Main Authors: Lee, Chun-Wei, 李俊葦
• Other Authors: 曾繁根
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/45271516520941997434

碩士 === 國立清華大學 === 工程與系統科學系 === 101 === General common biological detection: 1st Polymerase Chain Reaction, PCR). 2nd Enzyme-linked Immunosobent Assay, ELISA). but such methods biggest problem that requires a lot of time to get results, certainly not conducive to the urgent need of the results of the inspection body. The high surface-enhanced Raman scattering (SERS) can provide immediate, specific specificity without calibration, remote observation, the advantages of the specimen relatively few physical and chemical damage, prompting a lot of research team has focus it . The study of surface-enhanced Raman scattering (SERS) method to construct two mechanisms: 1st Surface-enhanced Raman scattering particles (AuPSBs or AgPSBs). 2nd AC Electroosmotic Chip. AuPSBs is etching polystyrene beads formation of nano-rough surface by oxygen plasma bombardment and deposit metal film on the surface, and does not have a the nano rough surface of metal coated particles Raman signal compared to about 5 times capacity; AC seepage chip is the regulation of voltage and frequency of suspended particulates manipulation electrodynamics, this method can be a tenfold improvement in signal strength within six minutes, and AC seepage can control the suspended particulates centralized location, this method can be resolved Raman scattering detection in the object to be tested positioning difficulties. Combining the above two mechanisms can be achieved in 2 minutes 50 times signal amplification capability, should be in the future utility in the rapid detection of biological or medical.