Protective effect of flavonoid compounds on di(2-ethylhexyl)phthalate (DEHP) induced injury in mouse liver FL83B cells

• Main Authors: Diana Lo, 盧琪琪
• Other Authors: Ming-Chang Wu
• Format: Others
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/87689763312788261142

碩士 === 國立屏東科技大學 === 熱帶農業暨國際合作系所 === 101 === Di(2-ethylhexyl)phthalate (DEHP) is one of the most widespread phthalate plasticizers. In May 2011, the illegal usage of DEHP in food and beverages caused a hazard to public in Taiwan. The liver is the key organ of metabolism and excretion and is continuously exposed to xenobiotics. The toxins absorbed from the intestinal tract gain access, first to the liver, resulting in a variety of liver ailments. Therefore, this study was intended to investigate the hepatoprotective effect of flavonoid compounds on DEHP-induced injury in mouse liver FL83B cells. The potency of structurally-distinct representative flavonoid compounds (silymarin, taxifolin and quercetin) for preventing DEHP-induced injury on liver FL83B cells was evaluated by studying cell viability, cell morphology, lactate dehydrogenase (LDH) activity, cell cycle analysis and lipid peroxidation. Results revealed that silymarin had high hepatoprotective activities and taxifolin had low hepatoprotective activities, while quercetin had no hepatoprotective activity. Silymarin, at the concentration of 25 µM, achieved the highest hepatoprotective effect. Besides reducing cell viability, silymarin was also found to reduce LDH activity and number of cells in S phase. Interestingly, DEHP exposure on HepG2 human carcinoma cell line and black dot-contaminated FL83B cells resulted in higher number of viable cell.